《植物生理学报》 2009, 45(12): 1155-1159
通信作者:王玉成;E-mail: wangyucheng1029@yahoo.com.cn;Tel: 0451-82190607
摘 要:
从二色补血草 cDNA 文库中分离出一个 V-H+-ATP 酶 C 亚基(LbVHA-C)基因全长 cDNA 序列。该基因全长为 729 bp。 其中, 开放读码框(ORF)为 498 bp, 编码 165 个氨基酸, 预测编码蛋白的分子量为 16.6 kDa, 理论等电点为 8.62。用实时定量 RT-PCR 方法进一步研究二色补血草在 NaCl、NaHCO3 和 NaCO3 胁迫下的不同时间内该基因表达模式的结果表明, NaCl 强烈诱导二色补血草叶组织中 LbVHA-C 基因的表达, 但其根部的表达变化不明显。NaHCO3 胁迫下LbVHA-C 基因在根部 组织的表达受抑制。Na2CO3 胁迫24 h 时的LbVHA-C 基因在根和叶部组织中的表达都强烈受抑制, 随后在根和叶组织的表 达量逐步升高。关键词:二色补血草; V-H+-ATP 酶 C 亚基基因; 实时定量 RT-PCR; 基因表达
收稿:2009-06-30 修定:2009-11-08
资助:黑龙江省青年科技专项(QC07C56)和教育部科学技术研究重点项目(107037 )。
Corresponding author: WANG Yu-Cheng; E-mail: wangyucheng1029@yahoo.com.cn; Tel: 0451-82190607
Abstract:
A novel vacuolar H+-ATPase gene (LbVHA-C) was cloned from a cDNA library of Limonium bicolor. Sequence analysis showed that the LbVHA-C gene was 729 bp in length, including 498 bp of open reading frame (ORF), and encodes a protein of 165 amino acids with a predicted molecular mass of 16.6 kDa and pI of 8.62. The expression of LbVHA-C gene of L. bicolor in response to NaCl, NaHCO3 and Na2CO3 were investigated by real-time RT-PCR. The result showed that the expression of LbVHA-C gene was strongly induced by NaCl in leaves of L. bicolor, but not highly changed in roots. Under NaHCO3 stress, the expression of LbVHAC gene was inhibited in roots. And the expression of LbVHA-C gene were also strongly inhibited in both roots and leaves under NaCO3 stress for 24 h, then the expression increased.Key words: Limonium bicolor; vacuolar H+-ATPase gene (LbVHA-C); real time RT-PCR; gene expression
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